Rat CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit
The Rat CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is a reliable and accurate tool for detecting levels of CK-MB in rat samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides precise and reproducible results, making it suitable for various research applications.CK-MB is an important enzyme that plays a key role in cardiac muscle health, particularly in diagnosing and monitoring myocardial infarction (heart attack). By measuring CK-MB levels, researchers and healthcare professionals can assess heart damage and track recovery progress in animal models, offering valuable insights for cardiovascular research and drug development efforts.
Product Name:
Rat CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit
SKU:
RTES00976
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
