The Mouse CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is specifically designed for the accurate measurement of CK-MB levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.Creatine Kinase-MB is an important enzyme in the heart that is released into the bloodstream following heart muscle damage, making it a valuable biomarker for detecting myocardial infarction and other cardiac-related conditions in mice. The Mouse CK-MB ELISA Kit provides researchers with a reliable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse CK-MB ELISA Kit from AssayGenie is a valuable resource for researchers working in the field of cardiovascular research and preclinical drug development in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
