The Phospho-TIFA-T9 Polyclonal Antibody (CABP1395) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically detects phosphorylation at the T9 site of the TIFA protein in human samples, making it ideal for use in Western blot applications. By targeting this phosphorylation site, researchers can gain valuable insights into the regulation and activation of TIFA in various cell types.TIFA, also known as TRAF-interacting protein with forkhead-associated domain, plays a key role in the activation of NF-κB and MAPK signaling pathways in response to bacterial and viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-TIFA-T9 Polyclonal Antibody
SKU:
CABP1395
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ADTEE
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
T2BP, T6BP, TIFAA, Phospho-TIFA-T9
Positive Sample:
TIFA knockout (KO) 293T treated with ADP-heptose after transfected with human TIFA
Cellular Localization:
Cytoplasm.
Calculated MW:
21kDa
Observed MW:
21kDa
This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response.
Purification Method
Affinity purification
Gene ID
92610
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from TIFA knockout (KO) 293T cells transfected with TIFA using Phospho-TIFA-T9 Rabbit pAb (CABP1395) at 1:1000 dilution incubated overnight at 4℃. TIFA knockout (KO) 293T cells transfected with TIFA were treated with ADP-heptose. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
