Phospho-IRS1-S1101 Polyclonal Antibody (CABP0954)

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SKU:
CABP0954
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Synonyms:
HIRS-1, Phospho-IRS1-S1101
Frequently bought together:

Description

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Phospho-IRS1-S1101 Polyclonal Antibody (CABP0954)

The Phospho-IRS1-S1101 Polyclonal Antibody (CABP0954) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphorylation of IRS1 at this site plays a critical role in insulin signaling pathways, making this antibody essential for studies related to metabolic disorders such as diabetes.This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of IRS1 at serine 1101, researchers can investigate the downstream effects on insulin signaling and glucose metabolism in various cell types.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.

Product Name:Phospho-IRS1-S1101 Polyclonal Antibody
SKU:CABP0954
Size:20μL, 100μL
Reactivity:Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:HSSE T
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HIRS-1, Phospho-IRS1-S1101
Positive Sample:C2C12 treated with PMA
Cellular Localization:Caveola, Cytoplasm, Cytosol, Nucleoplasm, Nucleus, Plasma Membrane.
Calculated MW:132kDa
Observed MW:180kDa

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Purification MethodAffinity purification
Gene ID3667
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S1101 Rabbit pAb (CABP0954) at 1:1000 dilution. C2C12 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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