The NDUFS4 Monoclonal Antibody (CAB22056) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the NDUSF4 protein, a subunit of complex I involved in electron transport and energy production in the mitochondria.Raised in mice, this monoclonal antibody is highly specific and sensitive for detecting NDUSF4 in various experimental settings, such as Western blotting and immunofluorescence. Its reliability and reproducibility make it an essential reagent for exploring the role of NDUSF4 in physiological and pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFS4 Monoclonal Antibody
SKU:
CAB22056
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54574
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4724
RRID
AB_3427930
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFS4 Rabbit mAb (CAB22056) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using NDUFS4 Rabbit mAb (CAB22056) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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