Description
Liquid Zinc Assay (BA0051) (BA0051)
The Liquid Zinc Assay (SKU: BA0051) provides a simple, direct and automation-ready method for measuring zinc directly in biological samples without any pretreatment. Zinc is an essential trace element required for the activity of more than 300 enzymes, the structure of many proteins and the control of genetic expression, and its status affects cell division, growth, differentiation and development. Zinc deficiency is commonly caused by low dietary intake and low bioavailability and is associated with conditions including malabsorption syndrome, chronic liver and renal disease, sickle cell disease, diabetes and malignancy. The method utilises a chromogen that forms a coloured complex specifically with zinc, with the intensity of the colour, measured at 425nm, directly proportional to the zinc concentration. Only 50 ยตL of sample is required, and the optimised formulation greatly enhances reagent and signal stability in cuvette or 96-well plate formats.
| Product Name: | Liquid Zinc Assay (BA0051) |
| SKU: | BA0051 |
| Detection Method: | Colorimetric |
| Detection Range: | 0.12 ยตM (0.78 ยตg/dL) to 10 ยตM (65 ยตg/dL) zinc in 96-well assay format |
| Sample Type: | Serum, plasma (no EDTA), urine, saliva, waste water and soil |
| Species Reactivity: | All |
| Assay Time: | 30 minutes |
| Kit Size: | 250 Assays |
| Equipment Required: | Microplate reader |
| Storage: | The kit is shipped at room temperature. Store Reagents B and C at -20ยฐC and other components at 4ยฐC. |
| Shelf Life: | 6 months after receipt |
| Shipping: | Room Temperature |
This kit measures zinc directly in biological samples without pretreatment. The method utilises a chromogen that forms a coloured complex specifically with zinc, with the intensity of the colour, measured at 425nm, directly proportional to the zinc concentration in the sample.
- Sensitive and accurate: uses 50 ยตL samples; linear detection range 0.12 ยตM (0.78 ยตg/dL) to 10 ยตM (65 ยตg/dL) zinc in 96-well assay format
- Simple and high-throughput: addition of a single working reagent and incubation for 30 minutes, readily automated for thousands of samples per day
- Improved reagent stability and versatility in cuvette or 96-well plate formats
- Low interference in biological samples: no pretreatments needed
- Direct assays: zinc in serum, plasma (no EDTA), urine and saliva
- Drug discovery/pharmacology: effects of drugs on zinc metabolism
- Environment: zinc determination in waste water, soil etc.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Sample preparation: ensure serum and plasma samples are clear and free of turbidity; remove precipitates by filtration or centrifugation. Dilute serum or plasma 5-fold (n = 5) in deionised water before assay. |
| 2 | Equilibrate all reagents to room temperature and vortex Reagents B and C. Prepare Working Reagent: for each assay well mix 200 ยตL Reagent A, 4 ยตL Reagent B and 4 ยตL Reagent C. |
| 3 | Prepare standards in deionised water and transfer 50 ยตL of the Zn2+ standards into wells of a clear flat-bottom 96-well plate. Transfer 50 ยตL Sample and Sample Blank (50 ยตL sample + 2 ยตL EDTA) into wells. Add 200 ยตL Working Reagent to all wells and tap lightly to mix. |
| 4 | Incubate 30 minutes at room temperature and read optical density at 425nm (range 420-426nm). |
Subtract blank OD (water, #5) from the standard OD values and plot โOD against Zn2+ standard concentrations. Calculate โOD for the Sample (= ODSAMPLE โ ODSAMPLE BLANK) and determine the Sample Zn2+ concentration from the standard curve by non-linear regression using a single-site saturation binding function. If Zn2+ exceeds 10 ยตM, dilute in deionised water, repeat the assay and multiply by the dilution factor. Conversion: 1 ยตM zinc equals 6.5 ยตg/dL or 0.065 ppm (65 ppb).
| Component | Quantity | Storage |
| Reagent A | 50 mL | 4ยฐC |
| Reagent B | 1 mL | -20ยฐC |
| Reagent C | 1 mL | -20ยฐC |
| EDTA (100 mM) | 1 mL | 4ยฐC |
| Zinc Standard (50 ยตM) | 1 mL | 4ยฐC |
