Human VRK3 Recombinant Protein (RPPB5135)

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RPPB5135

Description

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Product Name:Human VRK3 Recombinant Protein
Product Code:RPPB5135
Size:10µg
Species:Human
Target:VRK3
Synonyms:Serine/threonine-protein pseudokinase VRK3, vaccinia related kinase 3, inactive serine/threonine-protein kinase VRK3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The VRK3 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLDAK DGRLFNEQNF FQRAAKPLQV NKWKKLYSTP LLAIPTCMGF GVHQDKYRFL VLPSLGRSLQ SALDVSPKHV LSERSVLQVA CRLLDALEFL HENEYVHGNV TAENIFVDPE DQSQVTLAGY GFAFRYCPSG KHVAYVEGSR SPHEGDLEFI SMDLHKGCGP SRRSDLQSLG YCMLKWLYGF LPWTNCLPNT EDIMKQKQKF VDKPGPFVGP CGHWIRPSET LQKYLKVVMA LTYEEKPPYA MLRNNLEALL QDLRVSPYDP IGLPMVP

VRK3 is a member of the protein kinase superfamily. The VRK3 gene has replacements at a number of residues within the ATP binding motifs which in other kinases were proved to be required for catalysis in both human and mouse. In vitro assays show that VRK3 has no phosphorylation activity but probably holds its substrate binding capability. VRK3 is universally expressed in human tissues and its protein is restricts to the nucleus.

VRK3 Human Recombinant produced in E. coli is a single polypeptide chain containing 497 amino acids (1-474) and having a molecular mass of 55.3 kDa.VRK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.
UniProt Protein Details:
NCBI Summary:This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q8IV63
NCBI GenInfo Identifier:71164888
NCBI Gene ID:51231
NCBI Accession:NP_001020949.1
UniProt Secondary Accession:Q8IV63,Q502Y2, Q9P2V8, A6NEG5, A8KA53
UniProt Related Accession:Q8IV63
Molecular Weight:52 kD
NCBI Full Name:inactive serine/threonine-protein kinase VRK3 isoform 2
NCBI Synonym Full Names:VRK serine/threonine kinase 3
NCBI Official Symbol:VRK3��
NCBI Official Synonym Symbols:
NCBI Protein Information:inactive serine/threonine-protein kinase VRK3
UniProt Protein Name:Inactive serine/threonine-protein kinase VRK3
UniProt Synonym Protein Names:Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3
Protein Family:Inactive serine/threonine-protein kinase
UniProt Gene Name:VRK3��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8IV63
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