Human U2AF1 Recombinant Protein (RPPB5060)

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RPPB5060

Description

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Product Name:Human U2AF1 Recombinant Protein
Product Code:RPPB5060
Size:20µg
Species:Human
Target:U2AF1
Synonyms:Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliaryfactor small subunit, U2AF35, U2AFBP, FP793, RNU2AF1, RN.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:U2AF1 protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTILIQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKFRREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSRDRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF

U2AF1is a member of the splicing factor SR family. U2 auxiliary factor, comprising alarge and a smallsubunit, is a non-snRNP protein essential for the binding of U2 snRNP to thepre-mRNA branch site.U2AF1 is the small subunit which plays a vital role inboth constitutive and enhancer-dependent RNA splicing by directly mediating interactions betweenthe large subunit and proteins bound to the enhancers. U2 small nuclear RNAauxiliary factor 1 (U2AF1) is a protein-coding gene. Among the diseases whichare associated with U2AF1:Myoclonus Epilepsy and progressive Myoclonus Epilepsy.

U2AF1 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 263 amino acids (1-240 a.a) and havinga molecular mass of 30.3kDa.U2AF1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Spliceosome; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck

Molecular Function:RS domain binding; protein binding; metal ion binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription

NCBI Summary:This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q01081
NCBI GenInfo Identifier:267187
NCBI Gene ID:7307
NCBI Accession:Q01081.3
UniProt Secondary Accession:Q01081,Q701P4, Q71RF1,
UniProt Related Accession:Q01081
Molecular Weight:240
NCBI Full Name:Splicing factor U2AF 35 kDa subunit
NCBI Synonym Full Names:U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol:U2AF1��
NCBI Official Synonym Symbols:RN; FP793; U2AF35; U2AFBP; RNU2AF1��
NCBI Protein Information:splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit
UniProt Protein Name:Splicing factor U2AF 35 kDa subunit
UniProt Synonym Protein Names:U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit
Protein Family:Splicing factor
UniProt Gene Name:U2AF1��
UniProt Entry Name:U2AF1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q01081
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