Human U2AF1 Recombinant Protein (RPPB5060)
- SKU:
- RPPB5060
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q01081
Description
| Product Name: | Human U2AF1 Recombinant Protein |
| Product Code: | RPPB5060 |
| Size: | 20µg |
| Species: | Human |
| Target: | U2AF1 |
| Synonyms: | Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliaryfactor small subunit, U2AF35, U2AFBP, FP793, RNU2AF1, RN. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | U2AF1 protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTILIQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKFRREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSRDRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF |
U2AF1is a member of the splicing factor SR family. U2 auxiliary factor, comprising alarge and a smallsubunit, is a non-snRNP protein essential for the binding of U2 snRNP to thepre-mRNA branch site.U2AF1 is the small subunit which plays a vital role inboth constitutive and enhancer-dependent RNA splicing by directly mediating interactions betweenthe large subunit and proteins bound to the enhancers. U2 small nuclear RNAauxiliary factor 1 (U2AF1) is a protein-coding gene. Among the diseases whichare associated with U2AF1:Myoclonus Epilepsy and progressive Myoclonus Epilepsy.
U2AF1 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 263 amino acids (1-240 a.a) and havinga molecular mass of 30.3kDa.U2AF1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA splicing; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck Molecular Function:RS domain binding; protein binding; metal ion binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription |
| NCBI Summary: | This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q01081 |
| NCBI GenInfo Identifier: | 267187 |
| NCBI Gene ID: | 7307 |
| NCBI Accession: | Q01081.3 |
| UniProt Secondary Accession: | Q01081,Q701P4, Q71RF1, |
| UniProt Related Accession: | Q01081 |
| Molecular Weight: | 240 |
| NCBI Full Name: | Splicing factor U2AF 35 kDa subunit |
| NCBI Synonym Full Names: | U2 small nuclear RNA auxiliary factor 1 |
| NCBI Official Symbol: | U2AF1�� |
| NCBI Official Synonym Symbols: | RN; FP793; U2AF35; U2AFBP; RNU2AF1�� |
| NCBI Protein Information: | splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit |
| UniProt Protein Name: | Splicing factor U2AF 35 kDa subunit |
| UniProt Synonym Protein Names: | U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | U2AF1�� |
| UniProt Entry Name: | U2AF1_HUMAN |
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