The Human TSP-4 (Thrombospondin-4) ELISA Kit is specially designed for the precise measurement of thrombospondin-4 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.Thrombospondin-4 is a key protein involved in various biological processes, including cell adhesion, migration, and tissue remodeling. It is also implicated in conditions such as cardiovascular diseases, arthritis, and cancer, making it a valuable biomarker for studying these diseases and potential treatment options.With its reliable performance and user-friendly design, the Human TSP-4 ELISA Kit is a valuable tool for researchers seeking to explore the role of thrombospondin-4 in health and disease.
Product Name:
Human TSP-4 (Thrombospondin-4) ELISA Kit
SKU:
HUES02731
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
