Human Th1/Th2/Th17 Array (34 targets) (SARB0071)
- SKU:
- SARB0071
- Product Type:
- Protein Array
- Analytes:
- CD30 (TNFRSF8)
- Analytes:
- CD40 Ligand (TNFSF5)
- Analytes:
- CD40 (TNFRSF5)
- Analytes:
- GCSF
- Analytes:
- GITR (TNFRSF18)
- Analytes:
- GM-CSF
- Analytes:
- IFN-gamma
- Analytes:
- IL-1 beta (IL-1 F2)
- Analytes:
- IL-1 R1
- Analytes:
- IL-1 R2
- Analytes:
- IL-2
- Analytes:
- IL-4
- Analytes:
- IL-5
- Analytes:
- IL-6
- Analytes:
- IL-6 R
- Analytes:
- IL-10
- Analytes:
- IL-12 p40
- Analytes:
- IL-12 p70
- Analytes:
- IL-13
- Analytes:
- IL-17A
- Analytes:
- IL-17F
- Analytes:
- IL-17 RA
- Analytes:
- IL-21
- Analytes:
- IL-21 R
- Analytes:
- IL-22
- Analytes:
- IL-23
- Analytes:
- IL-28A (IFN-lambda 2)
- Analytes:
- MIP-3 alpha (CCL20)
- Analytes:
- gp130
- Analytes:
- TGF beta 1
- Analytes:
- TGF beta 3
- Analytes:
- TNF alpha
- Analytes:
- TNF beta (TNFSF1B)
- Analytes:
- TRANCE (TNFSF11)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0071 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 34 |
| Gene Symbols: | CCL20, CD40, CD40LG, CSF2, CSF3, IFNG, IFNL2, IL10, IL12A, IL12B|IL13, IL17A, IL17F, IL17RA, IL1B, IL1R1, IL1R2, IL2, IL21, IL21R, IL22, IL23A, IL4, IL5, IL6, IL6R, IL6ST, LTA, TGFB1, TGFB3, TNF, TNFRSF18, TNFRSF8, TNFSF11 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CD30 (TNFRSF8)
| CD40 Ligand (TNFSF5)
| CD40 (TNFRSF5)
| GCSF
| GITR (TNFRSF18)
|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-1 R1
| IL-1 R2
|
IL-2
| IL-4
| IL-5
| IL-6
| IL-6 R
|
IL-10
| IL-12 p40
| IL-12 p70
| IL-13
| IL-17A
|
IL-17F
| IL-17 RA
| IL-21
| IL-21 R
| IL-22
|
IL-23
| IL-28A (IFN-lambda 2)
| MIP-3 alpha (CCL20)
| gp130
| TGF beta 1
|
TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
| TRANCE (TNFSF11)
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Th1/Th2/Th17 Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
