Human Th1/Th2/Th17 Array (34 targets) (SARB0071)

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SKU:
SARB0071

Description

system_update_altDatasheet
Product Sku:SARB0071
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:34
Gene Symbols:CCL20, CD40, CD40LG, CSF2, CSF3, IFNG, IFNL2, IL10, IL12A, IL12B|IL13, IL17A, IL17F, IL17RA, IL1B, IL1R1, IL1R2, IL2, IL21, IL21R, IL22, IL23A, IL4, IL5, IL6, IL6R, IL6ST, LTA, TGFB1, TGFB3, TNF, TNFRSF18, TNFRSF8, TNFSF11
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
CD30 (TNFRSF8)
  • Gene Symbol: CD30
  • Gene ID: 943
  • Accession #: P28908
CD40 Ligand (TNFSF5)
  • Gene Symbol: CD40LG
  • Gene ID: 959
  • Accession #: P29965
CD40 (TNFRSF5)
  • Gene Symbol: CD40
  • Gene ID: 958
  • Accession #: P25942
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GITR (TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Gene ID: 8784
  • Accession #: Q9Y5U5
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
IL-1 R2
  • Gene Symbol: IL1R2
  • Gene ID: 7850
  • Accession #: P27930
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-17F
  • Gene Symbol: IL17F
  • Gene ID: 112744
  • Accession #: Q96PD4
IL-17 RA
  • Gene Symbol: IL17RA
  • Gene ID: 23765
  • Accession #: Q96F46
IL-21
  • Gene Symbol: IL21
  • Gene ID: 59067
  • Accession #: Q9HBE4
IL-21 R
  • Gene Symbol: IL21R
  • Gene ID: 50615
  • Accession #: Q9HBE5
IL-22
  • Gene Symbol: IL22
  • Gene ID: 50616
  • Accession #: Q9GZX6
IL-23
  • Gene Symbol: IL23A
  • Gene ID: 51561
  • Accession #: Q9NPF7
IL-28A (IFN-lambda 2)
  • Gene Symbol: IFNL2
  • Gene ID: 282616
  • Accession #: Q8IZJ0
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
gp130
  • Gene Symbol: Il6st
  • Gene ID: 3572
  • Accession #: P40189
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
TRANCE (TNFSF11)
  • Gene Symbol: TNFSF11
  • Gene ID: 8600
  • Accession #: O14788
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Th1/Th2/Th17 Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
CD30 (TNFRSF8)
Analytes:
CD40 Ligand (TNFSF5)
Analytes:
CD40 (TNFRSF5)
Analytes:
GCSF
Analytes:
GITR (TNFRSF18)
Analytes:
GM-CSF
Analytes:
IFN-gamma
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 R1
Analytes:
IL-1 R2
Analytes:
IL-2
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-6
Analytes:
IL-6 R
Analytes:
IL-10
Analytes:
IL-12 p40
Analytes:
IL-12 p70
Analytes:
IL-13
Analytes:
IL-17A
Analytes:
IL-17F
Analytes:
IL-17 RA
Analytes:
IL-21
Analytes:
IL-21 R
Analytes:
IL-22
Analytes:
IL-23
Analytes:
IL-28A (IFN-lambda 2)
Analytes:
MIP-3 alpha (CCL20)
Analytes:
gp130
Analytes:
TGF beta 1
Analytes:
TGF beta 3
Analytes:
TNF alpha
Analytes:
TNF beta (TNFSF1B)
Analytes:
TRANCE (TNFSF11)
Reactivity:
Human
Applications:
Multiplex Array
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