Human Immunology ELISA Kits 13
Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (HUES03534)
(No reviews yet)
Write a Review
- SKU:
- HUES03534
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- O14788(140~317aa)
- ELISA Type:
- Sandwich
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 9.38 pg/mL
- Range:
- 15.63-1000 pg/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit
The Human sRANKL (Soluble Receptor Activator of Nuclear Factor Kb Ligand) ELISA Kit is a powerful tool for the precise measurement of sRANKL levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.sRANKL is a vital protein involved in bone metabolism, regulating osteoclast differentiation and function. Dysregulation of sRANKL signaling is associated with bone-related diseases such as osteoporosis and rheumatoid arthritis, highlighting the importance of studying sRANKL levels in various pathological conditions. By using the Human sRANKL ELISA Kit, researchers can gain valuable insights into the role of sRANKL in bone health and disease, paving the way for the development of targeted therapies and diagnostic tools. With its advanced technology and reliable performance, this kit is an indispensable asset for investigating sRANKL biology and its implications in clinical settings.
| Product Name: | Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit |
| SKU: | HUES03534 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 9.38 pg/mL |
| Detection range: | 15.63-1000 pg/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
| Linearity: |
| ||||||||||||||||||||||||||||||||||||||||||
| Recovery: |
| ||||||||||||||||||||||||||||||||||||||||||
| Precision: |
| ||||||||||||||||||||||||||||||||||||||||||
| Uniport ID: | O14788 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Cardiovascular,Immunology |
Related Products
Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (AEKE04443)
system_update_altDatasheetsystem_update_altMSDS<
Mouse sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (AEKE06891)
system_update_altDatasheetsystem_update_altMSDS<
Dog sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (AEKE11740)
system_update_altDatasheetsystem_update_altMSDS<
Rat sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (RTFI01309)
Rat Signaling ELISA Kits 2
system_update_altDatasheetsystem_update_altMSDS<
Rat sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit (AEKE06889)
system_update_altDatasheetsystem_update_altMSDS<