Human NME2 Recombinant Protein (RPPB2590)

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RPPB2590
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Product Name:Human NME2 Recombinant Protein
Product Code:RPPB2590
Size:25µg
Species:Human
Target:NME2
Synonyms:Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B, EC 2.7.4.6, NDP kinase B, C-myc purine-binding transcription factor PUF, NDK B, NME2, puf, MGC111212.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NME2 protein solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

NME2 takes an important part in the synthesis of nucleoside triphosphates other than ATP. NME2 negatively controls Rho activity by interacting with AKAP13/LBC. NME2 acts as a transcriptional activator of the MYC gene. NME2 binds DNA non-specifically. NME2 is a heterodimeric enzyme functioning as a nucleoside diphosphate kinase. NME1 and NME2 contain 152 amino acids, A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases participate in the synthesis of nucleoside triphosphates, and NM23 is involved in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways.

NME2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.2kDa. The NME2 is purified by conventional chromatography.

UniProt Protein Function:NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; EC 2.7.13.3; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Kinase, other; EC 2.7.4.6; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: cytoplasm; cytosol; focal adhesion; lamellipodium; nucleus; ruffle

Molecular Function:nucleoside diphosphate kinase activity; protein binding; transcription factor activity

Biological Process: cell adhesion; integrin-mediated signaling pathway; negative regulation of apoptosis; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of keratinocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of epidermis development; regulation of transcription, DNA-dependent

NCBI Summary:Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
UniProt Code:P22392
NCBI GenInfo Identifier:127983
NCBI Gene ID:4831
NCBI Accession:P22392.1
UniProt Secondary Accession:P22392,Q1WM23, Q6LCT6, A8MWA3,
UniProt Related Accession:P22392
Molecular Weight:30,137 Da
NCBI Full Name:Nucleoside diphosphate kinase B
NCBI Synonym Full Names:NME/NM23 nucleoside diphosphate kinase 2
NCBI Official Symbol:NME2��
NCBI Official Synonym Symbols:PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2��
NCBI Protein Information:nucleoside diphosphate kinase B
UniProt Protein Name:Nucleoside diphosphate kinase B
UniProt Synonym Protein Names:C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2
UniProt Gene Name:NME2��
UniProt Entry Name:NDKB_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22392
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