Human NF-kB Array (34 targets) (SARB0060)

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SKU:
SARB0060
Product Type:
Protein Array
Analytes:
ASC (PYCARD)
Analytes:
BCL-10
Analytes:
CD40 (TNFRSF5)
Analytes:
cIAP-1 (BIRC2/hIAP-2)
Analytes:
cIAP-2
Analytes:
c-Rel
Analytes:
FADD
Analytes:
Fas (TNFRSF6/Apo-1)
Analytes:
IkB-alpha
Analytes:
IKB-b (NFKBIB)
Analytes:
IkB-epsilon (NF-kappa-BIE)
Analytes:
IKK-B
Analytes:
IKK-E (IKBKE)
Analytes:
IKK-gamma (NEMO/FIP-3)
Analytes:
IL-1 Ra (IL-1 F3)
Analytes:
IL-1 R1
Analytes:
IL-17 RA
Analytes:
IL-1 R5 (IL-18 R alpha)
Analytes:
IRAK1
Analytes:
IRF5
Analytes:
JNK2 (MAPK9 isoform alpha-1)
Analytes:
Lymphotoxin beta R (TNF RIII)
Analytes:
MyD88
Analytes:
NFKB1
Analytes:
nfkb2
Analytes:
NGFR (TNFRSF16)
Analytes:
p53
Analytes:
socs6
Analytes:
STING
Analytes:
TLR2
Analytes:
TNF RI (TNFRSF1A)
Analytes:
TNF RII (TNFRSF1B)
Analytes:
TRAIL R1 (TNFRSF10A/DR4)
Analytes:
TRAIL R2 (TNFRSF10B/DR5)
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0060
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:34
Gene Symbols:ASC, BCL10, BIRC2, BIRC3, CD40, CIS4, FADD, FAS, FIP3, IKBB|IKBE, IKBKB, IKBKE, IL17RA, IL18R1, IL1R1, IL1RN, IRAK, IRF5, JNK2, LTBR, LYT10, MYD88, NFKB1, NGFR, REL, TLR2, TMEM173, TNFRSF10A, TNFRSF10B, TNFRSF1A, TNFRSF1B, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
ASC (PYCARD)
  • Gene Symbol: ASC CARD5 PYCARD TMS1
  • Gene ID: 29108
  • Accession #: Q9ULZ3
BCL-10
  • Gene Symbol: BCL10 CIPER CLAP
  • Gene ID: 8915
  • Accession #: O95999
CD40 (TNFRSF5)
  • Gene Symbol: CD40 TNFRSF5
  • Gene ID: 958
  • Accession #: P25942
cIAP-1 (BIRC2/hIAP-2)
  • Gene Symbol: BIRC2 API1 IAP2 MIHB RNF48
  • Gene ID: 329
  • Accession #: Q13490
cIAP-2
  • Gene Symbol: BIRC3 API2 IAP1 MIHC RNF49
  • Gene ID: 330
  • Accession #: Q13489
c-Rel
  • Gene Symbol: REL
  • Gene ID: 5966
  • Accession #: Q04864
FADD
  • Gene Symbol: FADD GIG3 MORT1
  • Gene ID: 8772
  • Accession #: Q13158
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: FAS APT1 FAS1 TNFRSF6
  • Gene ID: 355
  • Accession #: P25445
IkB-alpha
  • Gene Symbol: N/A
  • Gene ID: 4792
  • Accession #: P25963
IKB-b (NFKBIB)
  • Gene Symbol: IKBB NFKBIB TRIP9
  • Gene ID: 4793
  • Accession #: Q15653
IkB-epsilon (NF-kappa-BIE)
  • Gene Symbol: IKBE NFKBIE
  • Gene ID: 4794
  • Accession #: O00221
IKK-B
  • Gene Symbol: IKBKB IKKB
  • Gene ID: 3551
  • Accession #: O14920
IKK-E (IKBKE)
  • Gene Symbol: IKBKE IKKE IKKI KIAA0151
  • Gene ID: 9641
  • Accession #: Q14164
IKK-gamma (NEMO/FIP-3)
  • Gene Symbol: FIP3 IKBKG NEMO
  • Gene ID: 8517
  • Accession #: Q9Y6K9
IL-1 Ra (IL-1 F3)
  • Gene Symbol: IL1RN IL1F3 IL1RA
  • Gene ID: 3557
  • Accession #: P18510
IL-1 R1
  • Gene Symbol: IL1R1 IL1R IL1RA IL1RT1
  • Gene ID: 3554
  • Accession #: P14778
IL-17 RA
  • Gene Symbol: IL17RA IL17R
  • Gene ID: 23765
  • Accession #: Q96F46
IL-1 R5 (IL-18 R alpha)
  • Gene Symbol: IL18R1 IL1RRP
  • Gene ID: 8809
  • Accession #: Q13478
IRAK1
  • Gene Symbol: IRAK IRAK1
  • Gene ID: 3654
  • Accession #: P51617
IRF5
  • Gene Symbol: IRF5
  • Gene ID: 3663
  • Accession #: Q13568
JNK2 (MAPK9 isoform alpha-1)
  • Gene Symbol: JNK2 MAPK9 PRKM9 SAPK1A
  • Gene ID: N/A
  • Accession #: P45984-2
Lymphotoxin beta R (TNF RIII)
  • Gene Symbol: LTBR D12S370 TNFCR TNFR3 TNFRSF3
  • Gene ID: 4055
  • Accession #: P36941
MyD88
  • Gene Symbol: MYD88
  • Gene ID: 4615
  • Accession #: Q99836
NFKB1
  • Gene Symbol: NFKB1
  • Gene ID: N/A
  • Accession #: P19838-2
nfkb2
  • Gene Symbol: LYT10 NFKB2
  • Gene ID: 4791
  • Accession #: Q00653
NGFR (TNFRSF16)
  • Gene Symbol: NGFR TNFRSF16
  • Gene ID: 4804
  • Accession #: P08138
p53
  • Gene Symbol: TP53 P53
  • Gene ID: 7157
  • Accession #: P04637
socs6
  • Gene Symbol: CIS4 SOCS4 SOCS6
  • Gene ID: 9306
  • Accession #: O14544
STING
  • Gene Symbol: TMEM173 ERIS MITA STING
  • Gene ID: 340061
  • Accession #: Q86WV6
TLR2
  • Gene Symbol: TLR2 TIL4
  • Gene ID: 7097
  • Accession #: O60603
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A TNFAR TNFR1
  • Gene ID: 7132
  • Accession #: P19438
TNF RII (TNFRSF1B)
  • Gene Symbol: TNFRSF1B TNFBR TNFR2
  • Gene ID: 7133
  • Accession #: P20333
TRAIL R1 (TNFRSF10A/DR4)
  • Gene Symbol: TNFRSF10A APO2 DR4 TRAILR1
  • Gene ID: 8797
  • Accession #: O00220
TRAIL R2 (TNFRSF10B/DR5)
  • Gene Symbol: TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 UNQ160/PRO186
  • Gene ID: 8795
  • Accession #: O14763
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human NF-kB Array C2 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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