Human Cytokine Array (54 targets) (SARB0043)
- SKU:
- SARB0043
- Product Type:
- Protein Array
- Analytes:
- Activin A
- Analytes:
- ALCAM (CD166)
- Analytes:
- CD80 (B7-1)
- Analytes:
- BMP-5
- Analytes:
- BMP-7
- Analytes:
- Cardiotrophin-1 (CT-1)
- Analytes:
- CD14
- Analytes:
- CXCL16
- Analytes:
- DR6 (TNFRSF21)
- Analytes:
- Endoglin (CD105)
- Analytes:
- ErbB3
- Analytes:
- E-Selectin
- Analytes:
- Fas Ligand (TNFSF6)
- Analytes:
- ICAM-2 (CD102)
- Analytes:
- IGF-2
- Analytes:
- IL-1 R2
- Analytes:
- IL-10 R beta
- Analytes:
- IL-13 R alpha 2
- Analytes:
- IL-18 BP alpha
- Analytes:
- IL-18 R beta (AcPL)
- Analytes:
- MMP-3
- Analytes:
- IL-2 R beta (CD122)
- Analytes:
- IL-2 R gamma (Common gamma Chain)
- Analytes:
- IL-21 R
- Analytes:
- IL-5 R alpha
- Analytes:
- IL-9
- Analytes:
- IP-10 (CXCL10)
- Analytes:
- LAP/TGF beta 1
- Analytes:
- Leptin R
- Analytes:
- LIF
- Analytes:
- L-Selectin (CD62L)
- Analytes:
- M-CSF R
- Analytes:
- MMP-1
- Analytes:
- MMP-13
- Analytes:
- MMP-9
- Analytes:
- MPIF-1 (CCL23)
- Analytes:
- NGFR (TNFRSF16)
- Analytes:
- PDGF-AA
- Analytes:
- PDGF-AB
- Analytes:
- PDGF R alpha
- Analytes:
- PDGF R beta
- Analytes:
- PECAM-1 (CD31)
- Analytes:
- Prolactin
- Analytes:
- SCF R (CD117/c-kit)
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0043 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 54 |
| Gene Symbols: | ALCAM, BMP5, BMP7, CCL23, CD14, CD80, CDH5, CSF1R, CTF1, CXCL10|CXCL12, CXCL16, ENG, ERBB3, FASLG, FLT4, ICAM2, IGF2, IL10RB, IL13RA2, IL18BP, IL18RAP, IL1R2, IL21R, IL2RB, IL2RG, IL5RA, IL9, INHBA, KDR, KIT, LEPR, LIF, MMP1, MMP13, MMP3, MMP9, NGFR, PDGFA, PDGFB, PDGFRA, PDGFRB, PECAM1, PRL, SELE, SELL, SIGLEC5, TEK, TGFA, TGFB1, TGFB2, TIE1, TIMP4, TNFRSF21 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Activin A
| ALCAM (CD166)
| CD80 (B7-1)
| BMP-5
| BMP-7
|
Cardiotrophin-1 (CT-1)
| CD14
| CXCL16
| DR6 (TNFRSF21)
| Endoglin (CD105)
|
ErbB3
| E-Selectin
| Fas Ligand (TNFSF6)
| ICAM-2 (CD102)
| IGF-2
|
IL-1 R2
| IL-10 R beta
| IL-13 R alpha 2
| IL-18 BP alpha
| IL-18 R beta (AcPL)
|
MMP-3
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-21 R
| IL-5 R alpha
|
IL-9
| IP-10 (CXCL10)
| LAP/TGF beta 1
| Leptin R
| LIF
|
L-Selectin (CD62L)
| M-CSF R
| MMP-1
| MMP-13
| MMP-9
|
MPIF-1 (CCL23)
| NGFR (TNFRSF16)
| PDGF-AA
| PDGF-AB
| PDGF R alpha
|
PDGF R beta
| PECAM-1 (CD31)
| Prolactin
| SCF R (CD117/c-kit)
| SDF-1 beta (CXCL12 beta)
|
Siglec-5 (CD170)
| TGF alpha
| TGF beta 2
| Tie-1
| Tie-2
|
TIMP-4
| VE-Cadherin (CDH5)
| VEGFR2
| VEGFR3
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C8 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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