Cytokines Recombinant Proteins

Human CLCF1 Recombinant Protein (RPPB0126)

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RPPB0126

Description

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Product Name:Human CLCF1 Recombinant Protein
Product Code:RPPB0126
Size:20µg
Species:Human
Target:CLCF1
Synonyms:Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, BSF-3, Novel neurotrophin-1, NNT-1, CLCF1, BSF3, CLC, NNT1, NR6, CISS2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:CLCF1 protein solution (1mg/ml) containing 20mM sodium citrate (pH 3.5), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF

Cardiotrophin-like cytokine factor 1 (CLCF1) is a member of the interleukin 6 family of cytokines, which are involved in cell signaling via phosphorylation of gp130. CLCF1 has a sequence of 225 amino acids with a 27 aa signal peptide, having a molecular mass of 22kDa in the mature form, and the maximum homology to cardiotrophin-1 and ciliary neurotrophic factor. CLCF1 is actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. Defects in the CLCF1 gene cause cold-induced sweating syndrome 2 (CISS2). The CISS2 syndrome is typified by profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine.

CLCF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (28-225 a.a) and having a molecular mass of 24.6kDa.CLCF1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: extracellular region

Molecular Function:ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; protein binding; protein heterodimerization activity; receptor binding

Biological Process: B cell differentiation; cell surface receptor linked signal transduction; JAK-STAT cascade; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of immunoglobulin production; positive regulation of isotype switching to IgE isotypes; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 2

NCBI Summary:This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
UniProt Code:Q9UBD9
NCBI GenInfo Identifier:56404673
NCBI Gene ID:23529
NCBI Accession:Q9UBD9.1
UniProt Secondary Accession:Q9UBD9,Q6NZA4, B4DNT4,
UniProt Related Accession:Q9UBD9
Molecular Weight:24,087 Da
NCBI Full Name:Cardiotrophin-like cytokine factor 1
NCBI Synonym Full Names:cardiotrophin-like cytokine factor 1
NCBI Official Symbol:CLCF1��
NCBI Official Synonym Symbols:CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1��
NCBI Protein Information:cardiotrophin-like cytokine factor 1
UniProt Protein Name:Cardiotrophin-like cytokine factor 1
UniProt Synonym Protein Names:B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1
Protein Family:Cardiotrophin-like cytokine factor
UniProt Gene Name:CLCF1��
UniProt Entry Name:CLCF1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UBD9
Research Area:
Cytokines
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