The Human CASP1 (Caspase-1) CLIA Kit is a highly sensitive and accurate assay designed for the detection of caspase-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with reliable and reproducible results, making it suitable for a wide range of research applications.Caspase-1 is a key enzyme involved in inflammation and the immune response, playing a critical role in mediating the inflammatory process and initiating pyroptosis. Dysregulation of caspase-1 activity has been linked to various inflammatory and autoimmune diseases, as well as infectious diseases.By accurately measuring caspase-1 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. The Human CASP1 (Caspase-1) CLIA Kit provides a valuable tool for studying caspase-1 biology and developing novel treatments for inflammatory and immune-related disorders.
Product Name:
Human CASP1 (Caspase 1) CLIA Kit
SKU:
HUES00016
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
