The Human Casp1 (Caspase-1) ELISA Kit is specifically designed for the accurate measurement of Caspase-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Caspase-1 is an important enzyme involved in the inflammatory response and immune system regulation. It plays a key role in the activation of the pro-inflammatory cytokines and processes such as pyroptosis. Dysregulation of Caspase-1 has been linked to various diseases, including autoimmune disorders, infectious diseases, and neurodegenerative conditions.This ELISA kit provides researchers with a valuable tool for studying the role of Caspase-1 in these diseases, as well as potential therapeutic interventions. The user-friendly format and accurate results make it a convenient and reliable choice for laboratories conducting immunoassays.
Product Name:
Human CASP1 (Caspase 1) ELISA Kit
SKU:
HUES01303
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
