HSPA4 Polyclonal Antibody (CAB22022)

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SKU:
CAB22022
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Reactivity:
Human, Mouse, Rat
Synonyms:
RY, APG-2, HSPH2, hsp70, hsp70RY, HEL-S-5a, HS24/P52, HSPA4
Frequently bought together:

Description

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HSPA4 Polyclonal Antibody (CAB22022)

The HSPA4 Polyclonal Antibody (CAB22022) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.HSPA4, also known as HSP70, plays a crucial role in maintaining protein homeostasis under stressful conditions, such as heat shock or oxidative stress. Its diverse functions make it a key player in various cellular processes, including protein folding, stabilization, and degradation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HSPA4 Polyclonal Antibody
SKU:CAB22022
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NLGQ PIKI RFQE SEER PKLF EELG KQIQ QYMK IISS FKNK EDQY DHLD AADM TKVE KSTN EAME WMNN KLNL QNKQ SLTM DPVV KSKE IEAK IKEL TSTC SPII SKPK PKVE PPKE EQKN AEQN GPVD GQGD NPGP QAAE QGTD TAVP SDSD KKLP EMDI D
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RY, APG-2, HSPH2, hsp70, hsp70RY, HEL-S-5a, HS24/P52, HSPA4
Positive Sample:293T, HeLa, Mouse brain, Rat brain
Cellular Localization:Cytosol, Extracellular Exosome, Mitochondrion, Nucleus.
Calculated MW:94kDa
Observed MW:110kDa

Predicted to enable ATP binding activity. Involved in chaperone-mediated protein complex assembly and protein insertion into mitochondrial outer membrane. Located in cytosol and extracellular exosome. Implicated in Chagas disease. Biomarker of chronic obstructive pulmonary disease; rheumatoid arthritis; type 2 diabetes mellitus; and ulcerative colitis.

Purification MethodAffinity purification
Gene ID3308
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HSPA4 Rabbit pAb (CAB22022) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of various lysates using HSPA4 Rabbit pAb (CAB22022) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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