Horse TOX-IgG (Toxoplasma-Immunoglobulin G) ELISA Kit (AEFI03840)
- SKU:
- AEFI03840
- Product Type:
- ELISA Kit
- Size:
- 96T
- Reactivity:
- Horse
- Detection Method:
- Indirect (Qualitative ELISA)
Description
Horse TOX-IgG (Toxoplasma-Immunoglobulin G) ELISA Kit
The Horse TOX-IgG (Toxoplasma-Immunoglobulin G) ELISA Kit from AssayGenie is a cutting-edge tool for precise measurement of Horse TOX-IgG levels in horse serum. The kit delivers exceptional sensitivity and specificity, enabling accurate quantification of equine anti-Toxoplasma antibodies across diverse sample cohorts. Horse TOX-IgG represents the immunoglobulin G fraction of the humoral immune response to Toxoplasma gondii infection in horses. This antibody class is a marker of either active or latent toxoplasmosis and is essential for serological diagnosis and epidemiological surveillance of this parasitic infection in equine populations, with particular relevance to reproductive complications and neuromuscular disease in affected animals.
Equipped with this ELISA kit, researchers can delve deep into the intricate mechanisms governed by Horse TOX-IgG and gain valuable insights into its involvement in different biological pathways. Unleash the potential of your horse research with the AssayGenie Horse TOX-IgG (Toxoplasma-Immunoglobulin G) ELISA Kit empowering innovation and discovery in the realm of molecular biology.
| Product Name: | Horse TOX-IgG (Toxoplasma-Immunoglobulin G) ELISA Kit |
| SKU: | AEFI03840 |
| Reactivity: | Horse |
| Detection Method: | Indirect (Qualitative ELISA) |
| Sample Type: | Serum |
| Storage: | 2-8°C for 12 months. |
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Plate Setup: Label wells for samples, 3 Negative Controls, 2 Positive Controls, and 1 blank well. |
| 2 | Controls & Sample Loading: Add Negative Control and Positive Control solutions to their respective wells. Add Sample Dilution Buffer followed by sample serum to sample wells. Tap gently to ensure mixing. |
| 3 | First Incubation & Wash: Seal the plate and incubate at 37°C for 30 minutes. Wash 5 times with Wash Buffer, allowing buffer to sit 1-2 minutes per wash. |
| 4 | HRP-Conjugate Addition & Wash: Add HRP-conjugated antigen solution to each well containing controls and samples. Incubate at 37°C for 30 minutes. Wash 5 times. |
| 5 | Color Development: Add TMB Substrate A and B to each well. Incubate at 37°C in the dark for 15 minutes. |
| 6 | Stop Reaction & Reading: Add Stop Solution and read absorbance at 450 nm immediately, using the blank well to set the zero reference. |
| Sample Type | Protocol |
| Serum | Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately. |
| Plasma | Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed. |
| Tissue Homogenate | Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant. |
| Cell Lysate | Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage |
| 96T | ||
| ELISA Microplate (Dismountable) | 8x12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| TOX-IgG Positive Control (Ready to use) | 1 ml | 2-8°C |
| TOX-IgG Negative Control (Ready to use) | 1 ml | 2-8°C |
| Sample Dilution Buffer | 12 ml | 2-8°C |
| HRP-Anti-Horse IgG Antibody | 12 ml | 2-8°C (Avoid direct light) |
| TMB Substrate | 6 ml | 2-8°C (Avoid direct light) |
| TMB Substrate | 6 ml | 2-8°C (Avoid direct light) |
| Stop Solution | 6 ml | 2-8°C |
| Wash Buffer(25X) | 50 ml | 2-8°C |
| Plate Sealer | 3 pieces | - |
| Technical Manual | 1 copy | - |
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