Cullin 1 Polyclonal Antibody (CAB21687)

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SKU:
CAB21687
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Applications:
IHC
Reactivity:
Human, Mouse, Rat
Synonyms:
CUL1, MGC149834, MGC149835, cullin-1, Cullin 1
Frequently bought together:

Description

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Cullin 1 Polyclonal Antibody (CAB21687)

The Cullin 1 Polyclonal Antibody (CAB21687) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting Cullin-1 in human samples and has been validated for use in Western blot and immunohistochemistry applications. By binding to the Cullin-1 protein, this antibody allows for the precise detection and analysis of Cullin-1 expression in various cell types.Cullin-1 plays a critical role in regulating the degradation of target proteins, thereby controlling important cellular processes such as cell cycle progression, signaling pathways, and DNA replication.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Cullin 1 Polyclonal Antibody
SKU:CAB21687
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KNPE EAEL EDTL NQVM VVFK YIED KDVF QKFY AKML AKRL VHQN SASD DAEA SMIS KLKQ ACGF EYTS KLQR MFQD IGVS KDLN EQFK KHLT NSEP LDLD FSIQ VLSS GSWP FQQS CTFA LPSE LERS YQRF TAFY ASRH SGRK LTWL YQLS KGEL VTN
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CUL1, MGC149834, MGC149835, cullin-1, Cullin 1
Positive Sample:Mouse brain, Rat brain
Cellular Localization:Cytosol, Nucleoplasm, Plasma Membrane.
Calculated MW:90kDa
Observed MW:90kDa

Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.

Purification MethodAffinity purification
Gene ID8454
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using Cullin 1 Rabbit pAb (CAB21687) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb (CAB21687) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb (CAB21687) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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