Immunology Antibodies 2

Cullin 1 Rabbit Polyclonal Antibody (CAB2136)

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SKU:
CAB2136
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
Cullin 1, CUL1, cullin-1, MGC149834, MGC149835
Research Use:
Immunology
Frequently bought together:

Description

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Cullin 1 Antibody (CAB2136)

The Cullin 1 Antibody (CAB2136) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the Cullin-1 protein, this antibody enables researchers to detect and analyze Cullin-1 levels in various cell types, making it ideal for studies in oncology and cell biology.Cullin-1 is a key component of the Cullin-RING E3 ubiquitin ligase complex, which plays a crucial role in targeting proteins for degradation via the ubiquitin-proteasome pathway.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Cullin 1 Antibody
SKU:CAB2136
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KNPE EAEL EDTL NQVM VVFK YIED KDVF QKFY AKML AKRL VHQN SASD DAEA SMIS KLKQ ACGF EYTS KLQR MFQD IGVS KDLN EQFK KHLT NSEP LDLD FSIQ VLSS GSWP FQQS CTFA LPSE LERS YQRF TAFY ASRH SGRK LTWL YQLS KGEL VTN
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Cullin 1, CUL1, cullin-1, MGC149834, MGC149835
Positive Sample:A-549, MCF7, NIH/3T3, K-562, C6, Mouse liver, Mouse brain, Rat testis
Cellular Localization:Cytosol, Nucleoplasm, Plasma Membrane.
Calculated MW:90kDa
Observed MW:80kDa

Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.

Purification MethodAffinity purification
Gene ID8454
RRIDAB_2764155
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Cullin 1 Rabbit pAb (CAB2136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb (CAB2136) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb (CAB2136) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb (CAB2136) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb (CAB2136) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using Cullin 1 Rabbit pAb (CAB2136). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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