ATAD3A/ATAD3B Polyclonal Antibody (CAB22703)

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SKU:
CAB22703
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Applications:
IF
Reactivity:
Human, Mouse, Rat
Synonyms:
TOB3, AAA-TOB3, ATAD3A/ATAD3B
Frequently bought together:

Description

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ATAD3A/ATAD3B Polyclonal Antibody (CAB22703)

The ATAD3A/ATAD3B Polyclonal Antibody (CAB22703) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.ATAD3A and ATAD3B are essential for maintaining mitochondrial homeostasis and are implicated in various cellular processes such as mitochondrial nucleoid organization, lipid metabolism, and mitochondrial fusion. Dysregulation of these proteins has been linked to mitochondrial dysfunction and diseases like neurodegenerative disorders and metabolic syndromes.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATAD3A/ATAD3B Polyclonal Antibody
SKU:CAB22703
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSWL FGVN KGPK GEGA GPPP PLPP AQPG AEGG GDRG LGDR PAPK DKWS NFDP TGLE RAAK AARE LEHS RYAK EALN LAQM QEQT LQLE QQSK LKEY EAAV EQLK SEQI RAQA EERR KTLS EETR QHQA RAQY QDKL ARQR YEDQ LKQQ QLLN EENL RKQE ESVQ KQEA MRRA TVER EMEL RHKN EMLR VETE ARAR AKAE RENA DIIR EQIR LKAS EHRQ TVLE SIRT AGTL FGEG FRAF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TOB3, AAA-TOB3, ATAD3A/ATAD3B
Positive Sample:HeLa, A-549, U-251MG
Cellular Localization:Mitochondrion Inner Membrane, Peripheral Membrane Protein.
Calculated MW:71/73kDa
Observed MW:68kDa

The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID55210 83858
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of U20S cells using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B Rabbit pAb (CAB22703) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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