Signal Transduction Antibodies 3

YWHAH Rabbit Polyclonal Antibody (CAB9079)

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SKU:
CAB9079
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
YWHA1, YWHAH
Research Use:
Signal Transduction
Frequently bought together:

Description

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YWHAH Antibody (CAB9079)

The YWHAH Antibody (CAB9079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to human YWHAH samples, making it an ideal choice for Western blot applications. The YWHAH protein, also known as 14-3-3 eta, is involved in the regulation of diverse cellular functions by binding to phosphorylated serine/threonine residues on target proteins. Its role in cell signaling pathways makes it an important target for research in the fields of cancer biology, neurobiology, and cell biology.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:YWHAH Antibody
SKU:CAB9079
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGDR EQLL QRAR LAEQ AERY DDMA SAMK AVTE LNEP LSNE DRNL LSVA YKNV VGAR RSSW RVIS SIEQ KTMA DGNE KKLE KVKA YREK IEKE LETV CNDV LSLL DKFL IKNC NDFQ YESK VFYL KMKG DYYR YLAE VASG EKKN SVVE ASEA AYKE AFEI SKEQ MQPT HPIR LGLA LNFS VFYY EIQN APEQ ACLL AKQA FDDA IAEL DTLN EDSY KDST LIMQ LLRD NLTL WTSD QQDE EAGE GN
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:YWHA1, YWHAH
Positive Sample:U-87MG, LO2, Raji, Jurkat, Mouse heart, Mouse kidney
Cellular Localization:Cerebellar Granule Cell To Purkinje Cell Synapse, Cytoplasm, Cytosol, Extracellular Exosome, Plasma Membrane, Presynapse.
Calculated MW:28kDa
Observed MW:28kDa

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.

Purification MethodAffinity purification
Gene ID7533
RRIDAB_2772935
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using YWHAH Rabbit pAb (CAB9079) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (CAB9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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