Cell Biology Antibodies 14

VPS18 Polyclonal Antibody (CAB16654)

(No reviews yet) Write a Review
SKU:
CAB16654
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PEP3, VPS18
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

VPS18 Polyclonal Antibody (CAB16654)

The VPS18 Polyclonal Antibody (CAB16654) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly reactive with human samples and is validated for use in Western blot applications. It binds to the VPS18 protein, allowing for the detection and analysis of VPS18 in various cell types, making it ideal for studies in cell biology and vesicle trafficking research.VPS18 is a crucial component of the ESCRT pathway, which is involved in the sorting and trafficking of proteins within the cell.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:VPS18 Polyclonal Antibody
SKU:CAB16654
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PEP3, VPS18
Positive Sample:A-549, mouse brain, rat brain
Cellular Localization:Autophagosome, Clathrin-Coated Vesicle, Corvet Complex, Early Endosome, Endosome, Glutamatergic Synapse, Late Endosome, Lysosome, Presynapse.
Calculated MW:110kDa
Observed MW:110kDa

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Purification MethodAffinity purification
Gene ID57617
RRIDAB_2772867
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using VPS18 Rabbit pAb (CAB16654) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 4min.
Product ImageImmunofluorescence analysis of C6 cells using VPS18 Rabbit pAb (CAB16654) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using VPS18 Rabbit pAb (CAB16654) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products