The VAV2 Monoclonal Antibody (CAB3548) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blot applications.Vav2 is a member of the Vav family of guanine nucleotide exchange factors (GEFs) that play a crucial role in the transduction of extracellular signals into the cell, ultimately influencing processes such as cell proliferation, differentiation, and migration.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
VAV2 Monoclonal Antibody
SKU:
CAB3548
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC2041
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FDVR DFGK VISA VSRL SLHS IAQN KGIR PFPS EETT ENDD DVYR SLEE LADE HDLG EDIY DCVP CEDG GDDI YEDI IKVE VQQP MIRY MQKM GMTE DDKR N
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7410
RRID
AB_2863085
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VAV2 Rabbit mAb (CAB3548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using VAV2 Rabbit mAb (CAB3548) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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