KO Validated Antibodies 1

Anti-VAT1 Antibody (CAB17077)[KO Validated]

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SKU:
CAB17077
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-VAT1 Antibody [KO Validated]
Antibody SKU:CAB17077
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human VAT1.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, U-87MG, 293T
Immunogen:A synthetic peptide of human VAT1.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10493
Uniprot:Q99536
Cellular Location:
Calculated MW:28kDa/34kDa/41kDa
Observed MW:42kDa
Synonyms:VAT1, VATI
Background:Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.
UniProt Protein Function:Possesses ATPase activity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
NCBI Summary:Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q99536
NCBI GenInfo Identifier:119581322
NCBI Gene ID:10493
NCBI Accession:EAW60918.1
UniProt Secondary Accession:Q99536,Q13035, Q5BKZ7, Q96A39, Q9BUT8, A8K345, B0AZP7 B4DPX4,
UniProt Related Accession:Q99536
Molecular Weight:28,341 Da
NCBI Full Name:vesicle amine transport protein 1 homolog (T californica), isoform CRA_a
NCBI Synonym Full Names:vesicle amine transport 1
NCBI Official Symbol:VAT1  
NCBI Official Synonym Symbols:VATI  
NCBI Protein Information:synaptic vesicle membrane protein VAT-1 homolog
UniProt Protein Name:Synaptic vesicle membrane protein VAT-1 homolog
Protein Family:Synaptic vesicle membrane protein
UniProt Gene Name:VAT1  
Anti-VAT1 Antibody [KO Validated] (CAB17077)Western blot analysis of extracts of various cell lines, using VAT1 antibody (CAB17077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Anti-VAT1 Antibody [KO Validated] (CAB17077)Western blot analysis of extracts from normal (control) and VAT1 knockout (KO) 293T cells, using VAT1 antibody (CAB17077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-VAT1 Antibody [KO Validated] (CAB17077)Immunofluorescence analysis of L929 cells using VAT1 antibody (CAB17077) at dilution of 1:100. Blue: DAPI for nuclear staining.
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