USP9Y Polyclonal Antibody (CAB20758)

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SKU:
CAB20758
Product Type:
Antibody
Isotype:
Horseradish peroxidase conjugated IgG
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Synonyms:
DFFRY, SPGFY2, USP9X/USP9Y
Frequently bought together:

Description

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USP9Y Polyclonal Antibody (CAB20758)

The USP9Y Polyclonal Antibody (CAB20758) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications.USP9Y, a member of the ubiquitin-specific protease family, is involved in various cellular processes including protein degradation and immune response regulation. Its role in modulating immune signaling pathways and inflammation makes it a promising target for studying immune-related diseases such as cancer and autoimmune disorders.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:USP9Y Polyclonal Antibody
SKU:CAB20758
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:YFLE RSHS ARMT LAKA CELC PEEE PDDQ DAPD EHEP SPSE DAPL YPHS PASQ YQQN NHVH GQPY TGPA AHHL NNPQ KTGQ RTQE NYEG NEEV SSPQ MKDQ
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DFFRY, SPGFY2, USP9X/USP9Y
Positive Sample:HeLa, Mouse testis, Rat brain
Cellular Localization:Cytoplasm, Cytosol, Nucleus.
Calculated MW:291kDa
Observed MW:291kDa

This gene is a member of the peptidase C19 family. It encodes a protein that is similar to ubiquitin-specific proteases, which cleave the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins.

Purification MethodAffinity purification
Gene ID8287
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using USP9X/USP9Y Rabbit pAb (CAB20758) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis tissue using USP9X/USP9Y Rabbit pAb (CAB20758) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of 293T cells using USP9X/USP9Y Rabbit pAb (CAB20758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HepG2 cells using USP9X/USP9Y Rabbit pAb (CAB20758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using USP9X/USP9Y Rabbit pAb (CAB20758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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