The USP33 Antibody (CAB16187) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in applications such as Western blotting.USP33 plays a crucial role in cellular signaling pathways, particularly those involved in cancer development, neurodegenerative diseases, and immune responses.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP33 Antibody
SKU:
CAB16187
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VDU1, USP33
Positive Sample:
HeLa, HepG2
Cellular Localization:
Cytoplasm, Golgi Apparatus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Perinuclear Region.
Calculated MW:
107kDa
Observed MW:
107kDa
This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms.
Purification Method
Affinity purification
Gene ID
23032
RRID
AB_2763638
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using USP33 Rabbit pAb (CAB16187) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Western blot analysis of lysates from HepG2 cells, using USP33 Rabbit pAb (CAB16187) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using USP33 Rabbit pAb (CAB16187) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using USP33 Rabbit pAb (CAB16187) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
