KO Validated Antibodies 2

Anti-TTC11 Antibody [KO Validated] (CAB19666)

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SKU:
CAB19666
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TTC11 Antibody [KO Validated]
Antibody SKU:CAB19666
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human TTC11.
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Raji, MCF7, Mouse kidney
Immunogen:A synthesized peptide derived from human TTC11.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:51024
Uniprot:Q9Y3D6
Cellular Location:Mitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein
Calculated MW:15kDa
Observed MW:17KDa
Anti-TTC11 Antibody [KO Validated] (CAB19666)Western blot analysis of extracts from normal (control) and TTC11 knockout (KO) HeLa cells, using TTC11 antibody (CAB19666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-TTC11 Antibody [KO Validated] (CAB19666)Western blot analysis of extracts of various cell lines, using TTC11 antibody (CAB19666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-TTC11 Antibody [KO Validated] (CAB19666)Immunohistochemistry of paraffin-embedded rat brain using TTC11 antibody (CAB19666) at dilution of 1:100 (40x lens).
Anti-TTC11 Antibody [KO Validated] (CAB19666)Immunohistochemistry of paraffin-embedded human lung cancer using TTC11 antibody (CAB19666) at dilution of 1:100 (40x lens).
Anti-TTC11 Antibody [KO Validated] (CAB19666)Immunohistochemistry of paraffin-embedded mouse kidney using TTC11 antibody (CAB19666) at dilution of 1:100 (40x lens).
Synonyms:CGI-135, TTC11, FIS1, TTC11 / FIS1, fission
Background:The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
UniProt Protein Function:FIS1: Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. Belongs to the FIS1 family.
UniProt Protein Details:

Protein type:Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: integral to mitochondrial outer membrane; integral to peroxisomal membrane; membrane; mitochondrion; peroxisome; protein complex

Molecular Function:protein binding; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; elevation of mitochondrial calcium ion concentration; mitochondrial fission; mitochondrial fragmentation during apoptosis; mitochondrial fusion; mitochondrion degradation; peroxisome fission; positive regulation of caspase activity; protein homooligomerization; protein targeting to mitochondrion; reduction of endoplasmic reticulum calcium ion concentration; release of cytochrome c from mitochondria

NCBI Summary:The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9Y3D6
NCBI GenInfo Identifier:33112470
NCBI Gene ID:51024
NCBI Accession:Q9Y3D6.2
UniProt Secondary Accession:Q9Y3D6,Q9BTP3,
UniProt Related Accession:Q9Y3D6
Molecular Weight:16,938 Da
NCBI Full Name:Mitochondrial fission 1 protein
NCBI Synonym Full Names:fission, mitochondrial 1
NCBI Official Symbol:FIS1  
NCBI Official Synonym Symbols:TTC11; CGI-135  
NCBI Protein Information:mitochondrial fission 1 protein
UniProt Protein Name:Mitochondrial fission 1 protein
UniProt Synonym Protein Names:FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11
Protein Family:Mitochondria fission 1 protein
UniProt Gene Name:FIS1  
UniProt Entry Name:FIS1_HUMAN
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