Cell Biology Antibodies 3

DNA topoisomerase I (TOP1) Rabbit Polyclonal Antibody (CAB12524)

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SKU:
CAB12524
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
TOPI, DNA topoisomerase I (TOP1)
Research Use:
Cell Biology
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Description

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DNA topoisomerase I (TOP1) Antibody (CAB12524)

The DNA topoisomerase I (TOP1) Antibody (CAB12524) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the TOP1 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for studies in molecular biology and cancer research.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:DNA topoisomerase I (TOP1) Antibody
SKU:CAB12524
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSGD HLHN DSQI EADF RLND SHKH KDKH KDRE HRHK EHKK EKDR EKSK HSNS EHKD SEKK HKEK EKTK HKDG SSEK HKDK HKDR DKEK RKEE KVRA SGDA
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TOPI, DNA topoisomerase I (TOP1)
Positive Sample:MCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary
Cellular Localization:Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:91kDa
Observed MW:120kDa

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Purification MethodAffinity purification
Gene ID7150
RRIDAB_2759364
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DNA topoisomerase I (TOP1) Rabbit pAb (CAB12524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver using DNA topoisomerase I (TOP1) Rabbit pAb (CAB12524) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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