Cell Biology Antibodies 6
Anti-TIMP2 Antibody (CAB1558)
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- SKU:
- CAB1558
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TIMP2 Antibody |
| Antibody SKU: | CAB1558 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIMP2 (NP_003246.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, 293T, Mouse spleen, Mouse lung, Mouse brain, Rat spleen, Rat lung, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIMP2 (NP_003246.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VSLD VGGK KEYL IAGK AEGD GKMH ITLC DFIV PWDT LSTT QKKS LNHR YQMG CECK ITRC PMIP CYIS SPDE CLWM DWVT EKNI NGHQ AKFF ACIK RSDG S |
| Gene ID: | 7077 |
| Uniprot: | P16035 |
| Cellular Location: | Secreted |
| Calculated MW: | 24kDa |
| Observed MW: | 26kDa |
| Synonyms: | TIMP2, CSC-21K, DDC8 |
| Background: | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. |
| UniProt Protein Function: | TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Cell development/differentiation; Inhibitor Chromosomal Location of Human Ortholog: 17q25 Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane Molecular Function:integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity Biological Process: response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging |
| NCBI Summary: | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P16035 |
| NCBI GenInfo Identifier: | 135854 |
| NCBI Gene ID: | 7077 |
| NCBI Accession: | P16035.2 |
| UniProt Secondary Accession: | P16035,Q16121, Q93006, Q9UDF7, |
| UniProt Related Accession: | P16035,Q96MC4 |
| Molecular Weight: | 24,399 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 2 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 2 |
| NCBI Official Symbol: | TIMP2 |
| NCBI Official Synonym Symbols: | DDC8; CSC-21K |
| NCBI Protein Information: | metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2 |
| UniProt Protein Name: | Metalloproteinase inhibitor 2 |
| UniProt Synonym Protein Names: | CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP2 |
| UniProt Entry Name: | TIMP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIMP2 antibody (CAB1558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using TIMP2 antibody (CAB1558) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using TIMP2 antibody (CAB1558) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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