The Timm29 Polyclonal Antibody (CAB20812) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By targeting the TIMM29 protein, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in mitochondrial biology and related fields.TIMM29, also known as Translocase of Inner Mitochondrial Membrane 29, plays a crucial role in protein translocation into the mitochondria, ultimately contributing to mitochondrial health and cellular metabolism.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Timm29 Polyclonal Antibody
SKU:
CAB20812
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Predicted to enable protein transporter activity. Predicted to be involved in protein insertion into mitochondrial inner membrane. Predicted to act upstream of or within protein transport. Predicted to be located in mitochondrial inner membrane and mitochondrial intermembrane space. Predicted to be part of TIM22 mitochondrial import inner membrane insertion complex. Orthologous to human TIMM29 (translocase of inner mitochondrial membrane 29).
Purification Method
Affinity purification
Gene ID
69773
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Timm29 Rabbit pAb (CAB20812) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 120s.
Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg Timm29 antibody (CAB20812). Western blot was performed from the immunoprecipitate using Timm29 antibody (CAB20812) at a dilution of 1:1000.
