Description
Antibody Name: | Anti-Timm29 Antibody (CAB20812) |
Antibody SKU: | CAB20812 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunoprecipitation |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 157-266 of mouse Timm29 (NP_848734.1). |
Application: | Western blotting, Immunoprecipitation |
Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
Reactivity: | Human, Mouse |
Positive Samples: | 293T, A-549, PC-3, Mouse liver |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 157-266 of mouse Timm29 (NP_848734.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | YLQP RWVD FPGR ILDV GFVG RWWI LQNR MHDC DIND DEFL HLPA HLRV VAPH QLHS EANE RLFE EKYK PIIL TDDQ VDQA LWEE QVLQ KERK DRLA LSEA DSLV QSDV SR |
Cellular Location: | |
Calculated MW: | 29kDa |
Observed MW: | 29KDa |
Synonyms: | TIM29, 1810026J23Rik |
Background: | Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. Required for the stability of the TIM22 complex and functions in the assembly of the TIMM22 protein into the TIM22 complex. May facilitate cooperation between TIM22 and TOM complexes by interacting with TOMM40. |
Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Timm29 antibody . Western blot was performed from the immunoprecipitate using Timm29 antibody at a dilition of 1:1000. | |
Western blot analysis of extracts of various cell lines, using Timm29 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s. |