The TFB1M Antibody (CAB15231) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the TFB1M protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component for investigations in the fields of mitochondrial biology and RNA modification.TFB1M is a crucial player in the maintenance of mitochondrial DNA integrity and the regulation of mitochondrial gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TFB1M Antibody
SKU:
CAB15231
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CGI75, mtTFB, CGI-75, mtTFB1, TFB1M
Positive Sample:
293T, A-549, MCF7, Mouse kidney, Rat heart
Cellular Localization:
Mitochondrion.
Calculated MW:
40kDa
Observed MW:
40kDa
The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID).
Purification Method
Affinity purification
Gene ID
51106
RRID
AB_2762126
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TFB1M Rabbit pAb (CAB15231) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
