The SRD5A2 Monoclonal Antibody (CAB19762) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the SRD5A2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component for studies in hormonal regulation, prostate cancer, and other androgen-related disorders.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SRD5A2 Monoclonal Antibody
SKU:
CAB19762
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2287
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
PC-3, Mouse liver, Mouse testis, Mouse kidney, Rat testis, Rat kidney
Cellular Localization:
Cell Body Fiber.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).
Purification Method
Affinity purification
Gene ID
6716
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SRD5A2 Rabbit mAb (CAB19762) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded mouse testis using SRD5A2 Rabbit mAb (CAB19762) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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