Cell Biology Antibodies 6

SPPL2A Rabbit Polyclonal Antibody (CAB15539)

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SKU:
CAB15539
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
IMP3, PSL2, IMD86, SPPL2A
Research Use:
Cell Biology
Frequently bought together:

Description

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SPPL2A Antibody (CAB15539)

The SPPL2A Antibody (CAB15539) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SPPL2A protein, this antibody enables the detection and analysis of SPPL2A in various cell types, making it ideal for studies in immunology and related fields.SPPL2A is a critical player in immune response regulation, particularly in the context of inflammatory and autoimmune diseases.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SPPL2A Antibody
SKU:CAB15539
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:200 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:IMP3, PSL2, IMD86, SPPL2A
Positive Sample:HeLa, Jurkat, Mouse brain, Rat small intestine
Cellular Localization:Late Endosome Membrane, Lumenal Side, Lysosome Membrane, Membrane, Multi-Pass Membrane Protein.
Calculated MW:58kDa
Observed MW:57kDa

This gene encodes a member of the GXGD family of aspartic proteases, which are transmembrane proteins with two conserved catalytic motifs localized within the membrane-spanning regions, as well as a member of the signal peptide peptidase-like protease (SPPL) family. This protein is expressed in all major adult human tissues and localizes to late endosomal compartments and lysosomal membranes. A pseudogene of this gene also lies on chromosome 15.

Purification MethodAffinity purification
Gene ID84888
RRIDAB_2762940
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using SPPL2A Rabbit pAb (CAB15539) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon using SPPL2A Rabbit pAb (CAB15539) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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