Cell Cycle Antibodies 1

SPIN1 Rabbit Polyclonal Antibody (CAB5853)

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SKU:
CAB5853
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
SPIN, TDRD24, SPIN1
Research Use:
Cell Cycle
Frequently bought together:

Description

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SPIN1 Antibody (CAB5853)

The SPIN1 Antibody (CAB5853) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Spindlin-1 in human samples, making it a reliable choice for various research applications, including Western blotting.Spindlin-1 is known to interact with histone proteins and play a role in epigenetic regulation, making it a key player in processes like cell differentiation and development.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SPIN1 Antibody
SKU:CAB5853
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MKTP FGKT PGQR SRAD AGHA GVSA NMMK KRTS HKKH RSSV GPSK PVSQ PRRN IVGC RIQH GWKE GNGP VTQW KGTV LDQV PVNP SLYL IKYD GFDC VYGL ELNK DERV SALE VLPD RVAT SRIS DAHL ADTM IGKA VEHM FETE DGSK DEWR GMVL ARAP VMNT WFYI TYEK DPVL YMYQ LLDD YKEG DLRI MPDS NDSP PAER EPGE VVDS LVGK QVEY AKED GSKR TGMV IHQV EAKP SVYF IKFD DDFH IYVY DLVK TS
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SPIN, TDRD24, SPIN1
Positive Sample:MCF7, SW480, 293T, Jurkat, HeLa, HepG2, SH-SY5Y, Mouse brain
Cellular Localization:Nucleus, Nucleolus.
Calculated MW:30kDa
Observed MW:32kDa

Enables methylated histone binding activity. Involved in positive regulation of Wnt signaling pathway; positive regulation of transcription, DNA-templated; and rRNA transcription. Located in cytosol and nucleus.

Purification MethodAffinity purification
Gene ID10927
RRIDAB_2766603
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SPIN1 Rabbit pAb (CAB5853) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using SPIN1 Rabbit pAb (CAB5853) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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