The SOD1 Monoclonal Antibody (CAB12537) is a high-quality antibody developed for reliable detection and analysis of target proteins. SOD1 is a critical antioxidant enzyme that plays a key role in protecting cells from oxidative stress, making it a key target for studies in oxidative stress, neurodegenerative diseases, and aging.This high-quality antibody, generated in rabbits, demonstrates high reactivity and specificity towards human samples, making it a reliable tool for Western blot applications. By specifically binding to SOD1, researchers can accurately measure the levels of this important enzyme in cell lysates, tissues, and other biological samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SOD1 Monoclonal Antibody
SKU:
CAB12537
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC51789
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
Purification Method
Affinity purification
Gene ID
6647
RRID
AB_2861671
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and SOD1 knockdown (KD) HeLa cells using [KD Validated] SOD1 Rabbit mAb (CAB12537) at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
