Cell Biology Antibodies 11

alpha-Synuclein Rabbit Polyclonal Antibody (CAB7215)

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SKU:
CAB7215
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PD1, NACP, PARK1, PARK4, α-Synuclein
Research Use:
Cell Biology
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Description

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alpha-Synuclein Antibody (CAB7215)

The alpha-Synuclein Antibody (CAB7215) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding specifically to alpha-synuclein, researchers can detect and analyze this protein in various cell types, allowing for important insights into the pathology of neurodegenerative diseases.Alpha-synuclein is a key player in the formation of Lewy bodies, abnormal protein aggregates found in the brains of individuals with Parkinson's disease and other synucleinopathies.

This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:alpha-Synuclein Antibody
SKU:CAB7215
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:TKEQ VTNV GGAV VTGV TAVA QKTV EGAG SIAA ATGF VKKD QLGK NEEG APQE GILE DMPV DPDN EAYE MPSE EGYQ DYEP EA
Tested Applications:WB IF/ICC ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PD1, NACP, PARK1, PARK4, α-Synuclein
Positive Sample:293T, Mouse brain, Rat brain, Mouse brain, Rat brain, 293T
Cellular Localization:Cell Junction, Cytoplasm, Membrane, Nucleus, Secreted, Cytosol, Synapse.
Calculated MW:14kDa
Observed MW:18kDa

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.

Purification MethodAffinity purification
Gene ID6622
RRIDAB_2767764
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using α-Synuclein Rabbit pAb (CAB7215) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using α-Synuclein Rabbit pAb (CAB7215) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of SH-SY5Y cells using α-Synuclein Rabbit pAb (CAB7215) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of Mouse brain tissue using α-Synuclein Rabbit pAb (CAB7215) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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