Cell Biology Antibodies 1

SNAP25 Rabbit Polyclonal Antibody (CAB0986)

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SKU:
CAB0986
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
SUP, RIC4, SEC9, SNAP, CMS18, RIC-4, SNAP-25, bA416N4.2, dJ1068F16.2, SNAP25
Research Use:
Cell Biology
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Description

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SNAP25 Antibody (CAB0986)

The SNAP25 Antibody (CAB0986) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to SNAP25, this antibody enables the detection and analysis of this protein in various cell types, making it an essential component for studies in neuroscience and neurology.SNAP25 is a key player in neurotransmitter release at synapses, making it a target of interest in research on neurological diseases such as Alzheimer's, Parkinson's, and epilepsy.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SNAP25 Antibody
SKU:CAB0986
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAED ADMR NELE EMQR RADQ LADE SLES TRRM LQLV EESK DAGI RTLV MLDE QGEQ LERI EEGM DQIN KDMK EAEK NLTD LGKF CGLC VCPC NKLK SSDA YKKA WGNN QDGV VASQ PARV VDER EQMA ISGG FIRR VTND AREN EMDE NLEQ VSGI IGNL RHMA LDMG NEID TQNR QIDR IMEK ADSN KTRI DEAN QRAT KMLG SG
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SUP, RIC4, SEC9, SNAP, CMS18, RIC-4, SNAP-25, bA416N4.2, dJ1068F16.2, SNAP25
Positive Sample:SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:Cell Junction, Cell Membrane, Cytoplasm, Lipid-Anchor, Perinuclear Region, Synapse, Synaptosome.
Calculated MW:23kDa
Observed MW:25kDa

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID6616
RRIDAB_2757505
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SNAP25 Rabbit pAb (CAB0986) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageWestern blot analysis of lysates from SH-SY5Y cells, using SNAP25 Rabbit pAb (CAB0986) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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