KO Validated Antibodies 1

[KO Validated] SMARCB1/SNF5 Rabbit Polyclonal Antibody (CAB5767)

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SKU:
CAB5767
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse, Rat
Synonyms:
RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, SMARCB1/SNF5
Research Use:
Cell Cycle
Frequently bought together:

Description

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[KO Validated] SMARCB1/SNF5 Antibody (CAB5767)

The [KO Validated] SMARCB1/SNF5 Antibody (CAB5767) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to SMARCB1, this antibody allows for the detection and analysis of this important protein in a variety of cell types, making it ideal for studies in epigenetics, cancer research, and developmental biology.SMARCB1, also known as INI1, is a key player in the SWI/SNF chromatin remodeling complex, which regulates gene expression by altering the structure of chromatin.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] SMARCB1/SNF5 Antibody
SKU:CAB5767
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LWRR LATV EERK KIVA SSHG KKTK PNTK DHGY TTLA TSVT LLKA SEVE EILD GNDE KYKA VSIS TEPP TYLR EQKA KRNS QWVP TLPN SSHH LDAV PCST T
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, SMARCB1/SNF5
Positive Sample:293T, NIH/3T3, Jurakt, K-562, Neuro-2a, Rat testis
Cellular Localization:Nucleus.
Calculated MW:44kDa
Observed MW:47kDa

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID6598
RRIDAB_2766521
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using SMARCB1/SNF5 Rabbit pAb (CAB5767) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human epithelioid sarcoma (ini-1 deletion) using SMARCB1/SNF5 Rabbit pAb (CAB5767) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg SMARCB1/SNF5 antibody (CAB5767). Western blot was performed from the immunoprecipitate using SMARCB1/SNF5 antibody (CAB5767) at a dilution of 1:1000.
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