KO Validated Antibodies 2

Anti-Smad4 Antibody [KO Validated] (CAB19116)

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SKU:
CAB19116
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Smad4 Antibody [KO Validated]
Antibody SKU:CAB19116
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Smad4
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HCT116, HepG2, C6, NIH/3T3, Mouse lung
Immunogen:A synthesized peptide derived from human Smad4
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4089
Uniprot:Q13485
Cellular Location:Cytoplasm, Nucleus
Calculated MW:65kDa
Observed MW:65kDa
Anti-Smad4 Antibody [KO Validated] (CAB19116)Western blot analysis of extracts from normal (control) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (CAB19116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Smad4 Antibody [KO Validated] (CAB19116)Western blot analysis of extracts of various cell lines, using Smad4 antibody (CAB19116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Smad4 Antibody [KO Validated] (CAB19116)Western blot analysis of extracts of various cell lines, using Smad4 antibody (CAB19116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-Smad4 Antibody [KO Validated] (CAB19116)Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] Smad4 Rabbit mAb (CAB19116) at dilution of 1:100 (40x lens).
Anti-Smad4 Antibody [KO Validated] (CAB19116)Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Smad4 antibody (CAB19116). Western blot was performed from the immunoprecipitate using Smad4 antibody (CAB19116) at a dilition of 1:1000.
Synonyms:DPC4, JIP, MADH4, MYHRS, Smad4, SMAD4
Background:This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]
UniProt Protein Function:SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; centrosome; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus

Molecular Function:collagen binding; identical protein binding; protein binding; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: developmental growth; axon guidance; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; palate development; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; regulation of binding; transcription, DNA-dependent; in utero embryonic development; neuron fate commitment; positive regulation of transforming growth factor beta receptor signaling pathway; gastrulation with mouth forming second; somite rostral/caudal axis specification; SMAD protein complex assembly; formation of anatomical boundary; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; regulation of hair follicle development; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; endoderm development

Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome

NCBI Summary:This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]
UniProt Code:Q13485
NCBI GenInfo Identifier:13959561
NCBI Gene ID:4089
NCBI Accession:Q13485.1
UniProt Secondary Accession:Q13485,A8K405,
UniProt Related Accession:Q13485
Molecular Weight:
NCBI Full Name:Mothers against decapentaplegic homolog 4
NCBI Synonym Full Names:SMAD family member 4
NCBI Official Symbol:SMAD4  
NCBI Official Synonym Symbols:JIP; DPC4; MADH4; MYHRS  
NCBI Protein Information:mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4
UniProt Protein Name:Mothers against decapentaplegic homolog 4
UniProt Synonym Protein Names:Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD4  
UniProt Entry Name:SMAD4_HUMAN
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