KO Validated Antibodies 1

[KO Validated] HSP27/HSPB1 Rabbit Polyclonal Antibody (CAB16332)

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SKU:
CAB16332
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Synonyms:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, B1
Research Use:
Immunology
Frequently bought together:

Description

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[KO Validated] HSP27/HSPB1 Antibody (CAB16332)

The [KO Validated] HSP27/HSPB1 Antibody (CAB16332) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the HSPB1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and molecular biology research.HSPB1, also known as heat shock protein 27 (Hsp27), is involved in protecting cells from stress-induced damage and maintaining protein homeostasis.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] HSP27/HSPB1 Antibody
SKU:CAB16332
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MTER RVPF SLLR GPSW DPFR DWYP HSRL FDQA FGLP RLPE EWSQ WLGG SSWP GYVR PLPP AAIE SPAV AAPA YSRA LSRQ LSSG VSEI RHTA DRWR VSLD VNHF APDE LTVK TKDG VVEI
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, B1
Positive Sample:HeLa
Cellular Localization:Cytoplasm, Nucleus, Cytoskeleton, Spindle.
Calculated MW:23kDa
Observed MW:27kDa

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Purification MethodAffinity purification
Gene ID3315
RRIDAB_2861725
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and HSP27/HSP27/HSPB1 knockout (KO) HeLa cells, using [KO Validated] HSP27/HSPB1 Rabbit pAb (CAB16332) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of lysates from HeLa cells, using [KO Validated] HSP27/HSPB1 Rabbit pAb (CAB16332) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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