Epigenetics & Nuclear Signaling Antibodies 3

SKIV2L2 Rabbit Polyclonal Antibody (CAB5576)

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SKU:
CAB5576
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
Dob1, Mtr4, SKIV2L2, fSAP118, KIAA0052
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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SKIV2L2 Antibody (CAB5576)

The SKIV2L2 Antibody (CAB5576) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples and has been validated for use in Western blot applications. By binding to the SKIV2L2 protein, this antibody allows for the detection and analysis of SKIV2L2 expression in various cell types, making it an essential tool for investigations in molecular biology and RNA biology.SKIV2L2 is a crucial component of the RNA exosome complex, which plays a vital role in degrading and processing RNA molecules within the cell.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SKIV2L2 Antibody
SKU:CAB5576
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MADA FGDE LFSV FEGD STTA AGTK KDKE KDKG KWKG PPGS ADKA GKRF DGKL QSES TNNG KNKR DVDF EGTD EPIF GKKP RIEE SITE DLSL ADLM PRVK VQSV ETVE GCTH EVAL PAEE DYLP LKPR VGKA AKEY PFIL DAFQ REAI QCVD NNQS VLVS AHTS AGKT VCAE YAIA LALR EKQR VIFT SPIK ALSN QKYR EMYE EFQD VGLM TGDV TINP TASC LVMT TEIL RSML YRGS EVMR EVAW VIFD EIHY MRDS ERGV VWEE TIIL LPDN VHYV FLSA TIPN ARQF AEWI CHLH KQPC
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Dob1, Mtr4, SKIV2L2, fSAP118, KIAA0052
Positive Sample:HeLa, 22RV1, 293T, U-87MG, Mouse testis, Mouse spleen
Cellular Localization:Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:118kDa
Observed MW:123kDa

Enables ATP binding activity and RNA helicase activity. Involved in RNA catabolic process; cellular response to DNA damage stimulus; and maturation of 5.8S rRNA. Located in nucleoplasm. Part of TRAMP complex and catalytic step 2 spliceosome. Colocalizes with nuclear exosome (RNase complex). Biomarker of amyotrophic lateral sclerosis.

Purification MethodAffinity purification
Gene ID23517
RRIDAB_2766353
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SKIV2L2 Rabbit pAb (CAB5576) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using SKIV2L2 Rabbit pAb (CAB5576) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis using SKIV2L2 Rabbit pAb (CAB5576) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SKIV2L2 antibody (CAB5576). Western blot was performed from the immunoprecipitate using SKIV2L2 antibody (CAB5576) at a dilution of 1:500.
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