Immunology Antibodies 2

SF3B2 Rabbit Polyclonal Antibody (CAB5875)

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SKU:
CAB5875
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
CFM, HFM, Cus1, SF3b1, SAP145, SF3B145, SF3b150, SF3B2
Research Use:
Immunology
Frequently bought together:

Description

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SF3B2 Antibody (CAB5875)

The SF3B2 Antibody (CAB5875) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the SF3B2 protein, this antibody enables precise detection and analysis in diverse cell types, making it an invaluable tool for investigations in molecular biology and gene expression studies.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SF3B2 Antibody
SKU:CAB5875
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SLGM PVGP NAHK VPPP WLIA MQRY GPPP SYPN LKIP GLNS PIPE SCSF GYHA GGWG KPPV DETG KPLY GDVF GTNA AEFQ TKTE EEEI DRTP WGEL EPSD EESS EEEE EEES DEDK PDET GFIT PADS GLIT PGGF SSVP AGME TPEL IELR KKKI EEAM DGSE TPQL FTVL PEKR TATV GGAM MGST HIYD MSTV MSRK GPAP ELQG VEVA LAPE ELEL DPMA MTQK YEEH VREQ QAQV EKED FSDM VAEH AAKQ KQKK RKAQ PQDS RGGS KKYK EFKF
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CFM, HFM, Cus1, SF3b1, SAP145, SF3B145, SF3b150, SF3B2
Positive Sample:HeLa, MCF7, SW620, A375, Mouse testis
Cellular Localization:Nucleus.
Calculated MW:100kDa
Observed MW:140kDa

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Purification MethodAffinity purification
Gene ID10992
RRIDAB_2766624
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SF3B2 Rabbit pAb (CAB5875) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat heart using SF3B2 Rabbit pAb (CAB5875) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse Intestine using SF3B2 Rabbit pAb (CAB5875) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-549 cells using SF3B2 Rabbit pAb (CAB5875).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
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