Metabolism Antibodies 1

HSP47/SERPINH1 Polyclonal Antibody (CAB13474)

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SKU:
CAB13474
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse
Synonyms:
CBP1, CBP2, OI10, gp46, AsTP3, HSP47, PIG14, PPROM, RA-A47, SERPINH2, HSP47/SERPINH1
Research Use:
Metabolism
Frequently bought together:

Description

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HSP47/SERPINH1 Polyclonal Antibody (CAB13474)

The HSP47/SERPINH1 Polyclonal Antibody (CAB13474) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.SERPINH1, also known as heat shock protein 47 (HSP47), plays a crucial role in collagen biosynthesis by assisting in the proper folding and stabilization of collagen triple helices. Dysregulation of SERPINH1 has been implicated in various pathological conditions, including fibrosis, connective tissue disorders, and cancer.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:HSP47/SERPINH1 Polyclonal Antibody
SKU:CAB13474
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SVSF ADDF VRSS KQHY NCEH SKIN FRDK RSAL QSIN EWAA QTTD GKLP EVTK DVER TDGA LLVN AMFF KPHW DEKF HHKM VDNR GFMV TRSY TVGV MMMH RTGL YNYY DDEK EKLQ IVEM PLAH KLSS LIIL MPHH VEPL ERLE KLLT KEQL KIWM GKMQ KKAV AISL PKGV VEVT HDLQ KHLA GLGL TEAI DKNK ADLS RMSG KKDL YLAS VFHA TAFE LDTD GNPF DQDI YGRE ELRS PKLF YADH PFIF LVRD TQSG SLLF IGRL VRPK GDKM RDEL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CBP1, CBP2, OI10, gp46, AsTP3, HSP47, PIG14, PPROM, RA-A47, SERPINH2, HSP47/SERPINH1
Positive Sample:NIH/3T3
Cellular Localization:Endoplasmic Reticulum Lumen.
Calculated MW:46kDa
Observed MW:46kDa

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.

Purification MethodAffinity purification
Gene ID871
RRIDAB_2760336
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using HSP47/SERPINH1 Rabbit pAb (CAB13474) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 Rabbit pAb (CAB13474) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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