Developmental Biology

SEMA4F Rabbit Polyclonal Antibody (CAB10432)

(No reviews yet) Write a Review
SKU:
CAB10432
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
S4F, SEMAM, SEMAW, M-SEMA, PRO2353, m-Sema-M, SEMA4F
Research Use:
Developmental Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

SEMA4F Antibody (CAB10432)

The SEMA4F Antibody (CAB10432) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to Semaphorin 4F, it allows for its detection and analysis in various cell types, making it an ideal tool for research in immunology and cancer.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SEMA4F Antibody
SKU:CAB10432
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RKTL QQRW TTFL KADL LCPG PEHG RASS VLQD VAVL RPEL GAGT PIFY GIFS SQWE GATI SAVC AFRP QDIR TVLN GPFR ELKH DCNR GLPV VDND VPQP RPGE CITN NMKL RHFG SSLS LPDR VLTF IRDH PLMD RPVF P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:S4F, SEMAM, SEMAW, M-SEMA, PRO2353, m-Sema-M, SEMA4F
Positive Sample:SH-SY5Y, HepG2, A-549, Mouse brain, Mouse liver, Mouse lung, Rat brain, Rat liver
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:84kDa
Observed MW:83kDa

This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID10505
RRIDAB_2757979
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SEMA4F Rabbit pAb (CAB10432) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEMA4F Rabbit pAb (CAB10432) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
View AllClose

0 Reviews

View AllClose

Related Products