Developmental Biology

Anti-SEMA4F Antibody (CAB10432)

(No reviews yet) Write a Review
SKU:
CAB10432
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SEMA4F Antibody
Antibody SKU:CAB10432
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HepG2, A-549, Mouse brain, Mouse liver, Mouse lung, Rat brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RKTL QQRW TTFL KADL LCPG PEHG RASS VLQD VAVL RPEL GAGT PIFY GIFS SQWE GATI SAVC AFRP QDIR TVLN GPFR ELKH DCNR GLPV VDND VPQP RPGE CITN NMKL RHFG SSLS LPDR VLTF IRDH PLMD RPVF P
Gene ID:10505
Uniprot:O95754
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:66kDa/83kDa
Observed MW:83kDa
Synonyms:SEMA4F, M-SEMA, PRO2353, S4F, SEMAM, SEMAW, m-Sema-M
Background:This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:SEMA4F: a single-pass type I membrane protein that may play a role in axon guidance.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: endoplasmic reticulum; integral to plasma membrane; membrane; plasma membrane

Biological Process: axon guidance; cell-cell signaling; nervous system development

NCBI Summary:This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:O95754
NCBI GenInfo Identifier:8134694
NCBI Gene ID:10505
NCBI Accession:O95754.2
UniProt Secondary Accession:O95754,Q542Y7, Q9NS35,
UniProt Related Accession:O95754
Molecular Weight:84kDa
NCBI Full Name:Semaphorin-4F
NCBI Synonym Full Names:ssemaphorin 4F
NCBI Official Symbol:SEMA4F  
NCBI Official Synonym Symbols:S4F; SEMAM; SEMAW; M-SEMA; PRO2353; m-Sema-M  
NCBI Protein Information:semaphorin-4F
UniProt Protein Name:Semaphorin-4F
UniProt Synonym Protein Names:Semaphorin-M; Sema M; Semaphorin-W; Sema W
Protein Family:Semaphorin
UniProt Gene Name:SEMA4F  
Anti-SEMA4F Antibody (CAB10432)Western blot analysis of extracts of various cell lines, using SEMA4F antibody (CAB10432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SEMA4F Antibody (CAB10432)Immunohistochemistry of paraffin-embedded human esophageal cancer using SEMA4F Rabbit pAb (CAB10432) at dilution of 1:200 (40x lens).
View AllClose

0 Reviews

View AllClose

Related Products